Advancing Human Plasma Proteome Fractionation: Integrating Next-Generation Chromatographic and Nanotechnology-Based Approaches for Clinical Biomarker Discovery in 2025

Abstract

Human plasma contains a vast and dynamic repertoire of proteins, many of which hold diagnostic and prognostic value for human diseases. However, the separation and characterization of low-abundance proteins remain a significant challenge in clinical proteomics. This study proposes the development of next-generation plasma protein fractionation techniques by integrating multi-dimensional chromatography, nanoparticle-assisted separation, and microfluidic systems. The aim is to optimize fractionation protocols for the detection of novel biomarkers associated with cancer, autoimmune disorders, and chronic inflammatory diseases. Unlike artificial intelligence-driven approaches, this research is entirely based on human plasma samples and experimental laboratory methods, ensuring originality, reproducibility, and adherence to ethical standards.